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. 2018 Nov 7;58(1):65–71. doi: 10.1021/acs.biochem.8b00925

Figure 1.

Figure 1

SMrT screen to identify membrane fission catalysts. (A) Schematic of supported membrane tubes (SMrT) and a supported lipid bilayer (SLB). (B) Tube radius distribution on PEG400- and PEG8000-functionalized glass coverslips. Data represent means ± the standard deviation (SD) for the indicated numbers of tubes. (C) Plot showing membrane-bound mEGFP-LactC2 with increasing bulk protein concentrations. Data represent the mean ± SD for n ≥ 10 tubes analyzed for each protein concentration. (D) Micrographs of DOPC/DOPS/p-Texas Red DHPE SMrT templates (49:50:1 molar ratio) acquired in the Texas Red channel in the absence and presence of excess LactC2 domain. The scale bar is 10 μm.