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. 2018 Dec 27;2018:7845629. doi: 10.1155/2018/7845629

Figure 5.

Figure 5

Characterization of vascular/cardiac oxidative stress and protective effects of macitentan cotherapy in ISMN-treated mice. DHE (1 μM) oxidative fluorescence microtopography was used to assess vascular oxidative stress (a). Representative staining images are shown below the densitometric quantification, and the green fluorescence represents autofluorescence of the cytoplasmic membranes. Levels of 3-NT-positive proteins in cardiac tissue were assessed by dot blot analysis and specific antibodies (b). Representative blots are shown below the densitometric quantification. Cardiac NADPH oxidase (NOX) activity was measured by the chemiluminescence probe lucigenin in the presence of NADPH (c). The data are mean ± SEM from at least 6 aortas (a), 8-9 hearts (b), and 5 pooled samples of at least 15 (c) mice per group. ∗p < 0.05 vs. control; #p < 0.05 vs. ISMN group.