Figure 3:

Mechanism of radiosensitization by eIF4E knockdown. In the following experiments MDA-MB-231 cells were transfected with siRNA specific to eIF4E (eIF4E KD) or non-targeted siRNA (Scramble). All experiments were carried out 72 hours post-transfection. A) Cell cycle phase distribution was determined. Values represent the mean of three independent experiments. B) Cells were irradiated with 2 or 4Gy and collected at the specified time; γH2AX foci were counted in at least 50 cells per condition. Values shown represent the means ± SE for 3 independent experiments, *p < 0.04 according to Student’s t test (eIF4E KD compared to scramble). C) Cells were irradiated (2 Gy) and collected at the specified time points. Cells were classified as being in mitotic catastrophe by the presence of nuclear fragmentation, which was defined as a single cell containing two or more distinct nuclear lobes. At least 50 cells per condition were scored. Values represent the mean ± SE for 3 independent experiments. *p< 0.04