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. Author manuscript; available in PMC: 2019 Sep 4.
Published in final edited form as: Anal Chem. 2018 Aug 14;90(17):10279–10285. doi: 10.1021/acs.analchem.8b02010

Figure 1.

Figure 1.

(A) miRNA detecting hydrogel microparticles are added directly to fixed cells or FFPE tissue section in experiments in this paper. (B) Target miRNA hybridizes to probes on the particles, biotinylated adapter sequence is ligated to the end of the bound miRNA target, and streptavidin-R-phycoerythrin (SAPE) binds to biotin to allow fluorescence quantification. (C) Calibration curve for particles detecting miR-21, and (D) example particles from calibration curve. Scale bar 50 µm. Limit of detection (LOD) is 2.0 amol.Error bars = 1 standard deviation.