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Multiplexed miRNA quantification from frozen and fixed Calu-6 cells. (A) miR-21 quantification from frozen and fixed Calu-6 cells. (B) miR-21 quantification from 10,000 fixed Calu-6 cells as a function of time after initial fixation. (C) Multiplexed quantification from 70,000 Calu-6 cells. (D) Representative particles of the five different miRNAs tested. Scale bar 50 µm. Error bars = 1 standard deviation.
