Fig. 1.

In a Collagen hydrogel incorporating hypoxia mimicking agent CoCl₂ (300 μM) or pHEMA coated culture plates, MCF7 breast cancer cells exhibited a dormant phenotype, which was reversible after treatment with CoCl₂ free growth media. Fluorescence images of MCF7 cells stained for Ki67 (red) and nuclei (blue) for untreated control, 3 day treatment with CoCl₂, 6 day treatment with CoCl₂ and 3 day treatment with CoCl₂ followed by 3 day recovery period in (a) Collagen hydrogels and (b) pHEMA coated culture plates and (c) quantification of Ki-67 status in these conditions. Scale bar = 200 μm. Figure taken from [51] and reprinted with permission of BioMed Central (Springer Nature)