Fig. 2.

DNA copy number gains and SP1 activation induce high SNHG6 expression in CRC. a GSEA results were plotted to visualize the correlation between the expression of SNHG6 and genes in adjacent chromosomal regions (CHR8Q11, CHR8Q12, CHR8Q13, CHR8Q21, CHR8Q22, CHR8Q23, and CHR8Q24). b SNHG6 genomic copy numbers in 30 CRC tissue samples. c SNHG6 genomic copy numbers in CRC cell lines and the normal colorectal epithelial cell line FHC. d Analysis of SP1 ChIP-seq, H3K4me3 ChIP-seq, and DnaseI-seq data of HCT-116 cells in the SNHG6 locus. e SNHG6 expression was detected by qRT-PCR in HCT-116 and HCT-8 cells transfected with the SP1 siRNAs or SP1 overexpression vector. f The correlation between the SP1 and SNHG6 expression levels were analyzed in 30 paired colorectal cancer samples (n = 30, r = 0.431, P = 0.017). g Luciferase reporter assays were used to determine the SP1 binding sites on the SNHG6 promoter region. h ChIP assays were performed to detect SP1 occupancy in the SNHG6 promoter region. **P < 0.01 and ***P < 0.001