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. 2019 Jan 9;17:15. doi: 10.1186/s12967-018-1751-9

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Technical and experimental validations. a Scatter plot showing the correlation between DNA methylation measurements performed by the Infinium MethylationEPIC BeadChip and by bisulfite pyrosequencing for 3 CpGs across the 11 subjects at the 3 time points (red, linear correlation estimation; grey, 1:1 line). b Box plots depicting the DNA methylation values of 3 CpGs measured by the Infinium MethylationEPIC BeadChip (top) and by bisulfite pyrosequencing (bottom). c Box plots indicating the DNA methylation values of the same 3 CpGs measured by bisulfite pyrosequencing in two independent cohorts, each comprising 9 children: measured (1) at 0 and 5 years of age and (2) at 5 and 10 years of age