Table 3.
Selection of differentially expressed genes in P. aeruginosa in co-culture versus mono-culture
| gene | FC a | annotation | functional category |
|---|---|---|---|
| Up-regulated genes | |||
| lldA | 14.13 | L-lactate dehydrogenase | metabolism |
| PA14_19690 | 7.77 | CidB, LrgB anti-holin protein | cell envelope |
| PA14_19680 | 6.51 | CidA, LrgA holin like protein | cell envelope |
| fdnH | 5.55 | nitrate inducible formate DH accessory protein | metabolism |
| PA14_64270 | 5.08 | Leu/Ile/Val-binding protein family signature | transport |
| PA14_55631 | 5.05 | 23S ribosomal RNA | RNA synthesis/modif. |
| PA14_62060 | 5.05 | 23S ribosomal RNA | RNA synthesis/modif. |
| PA14_61830 | 4.88 | tRNA-Met | RNA synthesis/modif. |
| PA14_63100 | 4.38 | cytochrome type D-lactate DH (4Fe-4S) | metabolism |
| narK1 | 3.95 | nitrite extrusion protein I | transport |
| PA14_24780 | 3.87 | Amoonium transporter | transport |
| lldD | 3.64 | L-lactate dehydrogenase | metabolism |
| PA14_60150 | 3.26 | tRNA-Lys | RNA synthesis/modif. |
| popN | 3.13 | type III secretion system | virulence |
| PA14_08670 | 3.01 | tRNA-Thr | RNA synthesis/modif. |
| PA14_08660 | 2.96 | tRNA-Gly | RNA synthesis/modif. |
| lldP | 2.94 | L-lactate permease | transport |
| ureE | 2.93 | urease accessory protein UreE | metabolism |
| glnA | 2.93 | glutamine synthetase | N-metabolism |
| mtr | 2.74 | Tryptophan permease | transport |
| PA14_36220 | 2.61 | Amino acid permease | transport |
| exsB | 2.56 | TTSS regulator | virulence |
| PA14_08210 | 2.52 | F-pyocin | Phage/pyocin |
| gdhA | 2.49 | glutamate dehydrogenase | N-metabolism |
| PA14_06890 | 2.36 | pyruvate kinase pyridoxal phosphate | metabolism |
| PA14_06930 | 2.32 | glutamine amidotransferase | metabolism |
| PA14_08070 | 2.30 | R-pyocin, phage tail protein | Phage/pyocin |
| PA14_06920 | 2.04 | class III pyridoxal phosphate aminotransferase | metabolism |
| Down-regulated genes | |||
| PA14_37310 | −20.35 | allophanate hydrolase subunit II putative | metabolism |
| PA14_37290 | −18.80 | allophanate hydrolase subunit I putative | metabolism |
| PA14_37270 | −18.65 | LamB/YcsF, carbohydrate/lactam utilization | metabolism |
| mmsB | −16.48 | 3-hydroxyisobutyrate dehydrogenase | aa metabolism |
| mmsA | −15.68 | methylmalonate-semialdehyde dehydrogenase | aa metabolism |
| PA14_37260 | −15.13 | OpdO, lactam/pyroglutamate uptake | transport |
| pchB | −14.79 | pyochelin synthesis | virulence |
| PA14_37250 | −12.58 | MFS transporter | transport |
| bkdA1 | −11.87 | 2-oxoisovalerate dehydrogenase subunit alpha | aa metabolism |
| gnuT | −11.53 | gluconate permease | transport |
| pchC | −11.32 | pyochelin synthesis | virulence |
| pchG | −10.48 | pyochelin synthesis | virulence |
| pchA | −9.97 | pyochelin synthesis | virulence |
| pchF | −9.56 | pyochelin synthesis | virulence |
| PA14_23010 | −9.47 | GltK, ATP-binding component of ABC transporter | transport |
| PA14_23000 | −9.37 | permease of ABC sugar transporter | transport |
| PA14_22990 | −9.19 | permease of ABC sugar transporter | transport |
| PA14_23030 | −8.63 | OprB, Glucose/carbohydrate porin | transport |
| bkdA2 | −8.05 | 2-oxoisovalerate dehydrogenase subunit beta | aa metabolism |
| bdhA | −5.81 | 3-hydroxybutyrate dehydrogenase | metabolism |
| bkdB | −4.93 | branched-chain alpha-keto acid dehydrogenase | metabolism |
| lpdV | −3.58 | dihydrolipoamide dehydrogenase | metabolism |
| fptA | −4.85 | pyochelin receptor | virulence |
| mexG | −4.32 | RND efflux pump | transport |
| mexH | −4.12 | RND efflux pump | transport |
| opmD | −3.49 | RND efflux pump | transport |
| mexI | −3.43 | RND efflux pump | transport |
| coxA | −3.90 | Cytochrome c oxidase subunit I | energy metabolism |
| coxB | −2.98 | Cytochrome c oxidase subunit II | energy metabolism |
| PA14_01310 | −2.64 | Cytochrome c oxidase assembly protein | energy metabolism |
| coIII | −2.15 | Cytochrome c oxidase subunit III | energy metabolism |
| napF | −2.97 | periplasmic nitrate reducatse | N-metabolism |
| napE | −2.45 | periplasmic nitrate reducatse | N-metabolism |
aFC fold change; values are the average of three replicates after normalization