Fig. 1.

HicB prevents the toxicity of HicA in E. coli. E. coli strain MG1655 containing pBBara (squares) or pBB-HicA (circles) were precultured in LB medium containing 10 mM glucose to an OD_570nm of 0.2. After centrifugation, cells were suspended in LB medium containing 10 mM glucose (black symbols) or 10 mM arabinose (white symbols). The growth of the strains was then followed for 3 h and estimated using either turbidity (OD_570nm, a) or viable cell counts (log (CFU/mL), b). The panel c corresponds to the numeration of the E. coli MG1655 cells containing pBBara + pNDM (A), pBB-HicA + pNDM (B), pBB-HicA + pNDM-RHH (C), pBB-HicA + pNDM-HicB (D), pBB-HicA + pNDM-COG (E) after 2 h of arabinose induction in LB medium, the details of the procedure were as in A. Tenfold dilutions were spotted on a LB plate and incubated for 48 h