Table 2.
Plasmids used in this study
| Names | Genotypes | References |
|---|---|---|
| pBAD24 | ori ColE1 araC, PBAD bla | [49] |
| pNDM220 | Mini R1, bla, lacIq, PA1/O4/O3 | [50] |
| pBBR1-MCS2 | ori pBBR1, lacZα, aphA | [40] |
| pET22b(+) | ori ColE1, bla, lacI, T7p | Novagen |
| pKT25 | ori P15A, aac, T25 | [37] |
| pKNT25 | ori P15A, aac, T25 | [37] |
| pUT18 | ori ColE1 bla, T18 | [37] |
| pUT18C | ori ColE1 bla, T18 | [37] |
| pKT25-Zip | ori P15A, aac, T25:: Zip | [37] |
| pUT18-Zip | ori ColE1 bla, T18:: Zip | [37] |
| pBBara | pBBR1-MCS2, araC, PBAD | |
| pBB-HicA | pBBR1-MCS2, araC, PBAD -hicA | This study |
| pET-HicB | pET22 hicB 6 his | This study |
| pET-RHH | pET22 RHH1 6 his | This study |
| pET-COG | pET22 COG 6 his | This study |
| pETHicAStrepHicBHis | pET22 synthetic operon | This study |
| pNDM-HicB | pNDM220 hicB | This study |
| pNDM-COG | pNDM220 NH2 fragment of HicB (COG1598) | This study |
| pNDM-RHH | pNDM220 COOH fragment of HicB (RHH) | This study |
| pKThicA | pKT25 hicA | This study |
| pKThicB | pKT25 hicB | This study |
| pNThicA | pKNT25 hicA | This study |
| pNThicB | pKNT25 hicB | This study |
| pUThicA | pUT18 hicA | This study |
| pUThicB | pUT18 hicB | This study |
| pUChicA | pUT18C hicA | This study |
| pUChicB | pUT18C hicB | This study |
| pKTCOG | pKT25 COG1598 | This study |
| pNTCOG | pKNT25 COG1598 | This study |
| pKTRHH | pKT25 RHH1 | This study |
| pNTRHH | pKNT25 RHH1 | This study |
| pUTCOG | pUT18 COG1598 | This study |
| pUCCOG | pUT18C COG1598 | This study |
| pUTRHH | pUT18 RHH1 | This study |
| pUCRHH | pUT18C RHH1 | This study |