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. 2019 Jan 9;19:16. doi: 10.1186/s12870-018-1597-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — ZmGPDH1 encodes a cytosol-targeted protein with GPDH activities. a Subcellular localization of ZmGPDH1. Confocal microscopy observation of pBI121-ZmGPDH1::GFP or pBI121-GFP in transgenic Arabidopsis mesophyll protoplasts. Bars = 10 μm. b Coomassie-stained 12% SDS-PAGE of cell extract from (Lane 1) Rosetta (DE3) Escherichia coli strain and (Lane 2–5) DE3 expressing His-tagged ZmGPDH1 protein. c Coomassie-stained 12% SDS-PAGE of (Lane 1) purified ZmGPDH1 fusion protein. d Western blot analysis of (Lane 1) purified ZmGPDH1 fusion protein using anti-6 × His antibody as probe. e The kinetic properties of ZmGPDH1 with regard to DHAP. f GPDH enzyme activities from purified ZmGPDH1 fusion protein. The reaction was performed in the presence (+) and absence (−) of NADH and DHAP, respectively