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. 2019 Jan 10;38:15. doi: 10.1186/s13046-018-0986-x

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — RER1 promotes oncogenicity of PC cells in vitro and in vivo. (a) Overexpression or knockdown of RER1 in Mia PaCa-2 cells were determined by qRT-PCR and Western blotting. (b) Overexpression or knockdown of RER1 in PANC-1 cells were determined by qRT-PCR and Western blotting. (c) CCK-8 assay showed cell survival of RER1 overexpression or knockdown Mia PaCa-2 cells. (d) CCK-8 assay showed cell survival of RER1 overexpression or knockdown PANC-1 cells (e and f) The percentages of BrdU positive Mia PaCa-2 and PANC-1 cells were determined. (g and h) Colony formation assay was conducted to assess cell survival of Mia PaCa-2 and PANC-1 cells (i and j) Cell cycle analysis of Mia PaCa-2 and PANC-1 cells were assessed by flow cytometry after staining with PI. The percentages of cells in three phases are shown in histogram. (k) Tumor growth curve of PANC-1-pSin-vec and PANC-1-pSin-RER1 cells implanted into the flank of male nude mice. (l) Cell proliferation was measured by Ki-67 staining assay. The data represent the mean ± SD from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 (Two-way ANOVA for c and d, student’s t-test for others)