Fig. 6.
Hypoxia induced PC progression and CSC phenotype are partially dependent on RER1. (a-c) CCK-8, colony formation and BrdU incorporation assays show viability of MIA PaCa-2 cells under normoxic culture, hypoxic culture or hypoxic culture with RER1 siRNA transfection (21%O2, 1%O2 or 1%O2 + si-RER1). (d) Cell cycle analysis of MIA PaCa-2 cells under normoxic culture, hypoxic culture or hypoxic culture with RER1 siRNA transfection (21%O2, 1%O2 or 1%O2 + si-RER1) were assessed by flow cytometry after staining with PI. (e-f) Scratch wound assay and Transwell assays were performed to determine the migration and invasion of MIA PaCa-2 cells under normoxic culture, hypoxic culture or hypoxic culture with RER1 siRNA transfection (21%O2, 1%O2 or 1%O2 + si-RER1). (g) Tumorsphere formation of MIA PaCa-2 cells under normoxic culture, hypoxic culture or hypoxic culture with RER1 siRNA transfection (21%O2, 1%O2 or 1%O2 + si-RER1). (h) CD133 positive cell population in MIA PaCa-2 cells under normoxic culture, hypoxic culture or hypoxic culture with RER1 siRNA transfection (21%O2, 1%O2 or 1%O2 + si-RER1) was determined by flow cytometry. Results are presented in histogram. The data represent the mean ± SD from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 (Two-way ANOVA for a, student’s t-test for others)