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. 2019 Jan 9;12:4. doi: 10.1186/s13041-018-0422-3

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Laser microdissection (LMD) enables isolation of lumbar motor neurons. a Nissl-stained axial sections of the lumbar spinal cord before (lower left image) and after microdissection (lower right image) of the boxed areas in the upper image. b The identity of neuronal cells isolated by LMD were confirmed by their expression of the motor neuron marker ChAT and not oligodendrocyte (CNPase) or astrocyte (GFAP) markers. Scale bars: 100 μm (a, upper panel); 20 μm (a, lower panels)