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. 2019 Jan 9;12:4. doi: 10.1186/s13041-018-0422-3

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Gene expression of lumbar motor neurons reveal decreased neuronal activity in the chronic phase of SCI. A: The mRNA expression of neuronal activity markers c-fos and CaMK2a in lumbar motor neurons isolated by LMD from the Naive and Tx3m groups (n = 8 mice per group). B: Immunohistochemistry for the neuronal activity markers c-fos (red) and NeuN (green) in the lumbar motor neurons, with nuclear counterstain in Hoechst (blue). The lower right images are magnifications of the boxed areas. *P < 0.05, Wilcoxon rank sum test (A). Data are presented as the mean ± SEM. Scale bars: 100 μm (B); 10 μm (B-a and B-b in the boxed areas)