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. 2019 Jan 9;12:4. doi: 10.1186/s13041-018-0422-3

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — The expression of the acetylcholine-related molecules and their axonal transportation are upregulated in motor neurons in the chronic phase of SCI. a mRNA expression of acetylcholine-related molecules in lumbar motor neurons isolated by LMD in the Naive and Tx3m group (n = 8 mice per group). b Immunohistochemistry for choline acetyltransferase in the lumbar motor neurons stained with ChAT (red), NeuN (green), and Hoechst (blue). Significantly larger number of ChAT-positive boutons are observed in the Tx3m group compared to the Naive group. *P < 0.05, n.s. = not significant (P > 0.05), Wilcoxon rank sum test (a). Data are presented as the mean ± SEM. Scale bars: 100 μm (b, left two panels); 20 μm (b, right two panels)