Fig. 1.

IL-5 is reduced in established mouse TH2 cells upon treatment with 4μ8c. D10 cells were rested in complete T cell media for 24 h at 37 °C. The cells were then left un-stimulated (NS) or stimulated with PMA and ionomycin (PI) or plate-bound α-CD3 and α-CD28 in the presence or absence (−) of 4μ8c for 24 h. a As a control the level of spliced xbp1 mRNA was measured by qRT-PCR, as 4μ8c blocks the ability of IRE1α to cleave xbp1. The data shown is the fold change in reduction of treated vs. untreated after normalizing to the ns control for five experiments. The supernatants were harvested, and ELISA was performed from these samples as shown in B and C. b The data shown is from six experiments where cells were re-stimulated with PMA and ionomycin in the presence or absence (−) of 4μ8c. c The data shown is for five experiments where the cells were re-stimulated with plate-bound antibodies in the presence or absence (−) of 4μ8c. The standard error, upper and lower bars, and the mean, middle bar, is shown in all graphs. Hypothesis testing was done by Student’s T test unpaired, Welch’s correction (p value < 0.05)