Fig. 3.

The reduction of IL-5 in 4μ8c treated cells is not due to changes in mRNA levels or stability. D10 cells were treated as in Fig. 1. a RNA was converted to cDNA and then amplified via qRT-PCR. The results show the relative fold change to the no stimulated sample. The data is an average of six experiments for the PMA and ionomycin stimulated samples (black bars) and five for the plate-bound stimulated ones (white bars). The standard error is graphed. b D10 cells were rested and then stimulated in the presence of 4μ8c for 24 h. The cells were then treated with actinomycin D and harvested at times 0, 10, 30, 60, and 90 min after treatment. RNA was isolated and qRT-PCR performed. The samples were normalized to the time zero point of actinomycin D treatment. The data were graphed on a semi-log scale and are the average of four experiments. The error bars represent the standard error of the mean. c Protein was isolated from cells treated as in A and immunoblotted with GATA-3 and β-actin antibody. The data is representative of three experiments