Fig. 3.

The effect of eCS on NF-κB activity. a Pre-treated with eCS for 16 h, RAW 264.7 cells were further treated with TLR4 specific LPS (100 ng/ml) for 30 min. Nuclear proteins were fractionated and analyzed by immunoblotting for p65 RelA, a key subunit of NF-κB. The membrane was stripped and blotted for Lamin A/C as for an internal control for nuclear proteins. Each band on the blots was analyzed by ImageJ, a densitometric analysis program (b). The relative levels of nuclear p65 RelA were calculated over Lamin A/C. ***P was less than 0.0001, compared to the LPS-treated control. Data are presented as the mean ± SEM of 3 measurements. At least, two more similar experiments were performed independently and a representative result is shown