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. 2018 Dec 11;17(2):1476–1485. doi: 10.3892/etm.2018.7084

Figure 3.

Figure 3.

(A) TargetScan software predicted that FZD4 was a target gene of miR-101. (B) The binding sequences of miR-101 within the WT or MT 3′UTR of FZD4 are indicated. (C) Luciferase reporter gene assay was then conducted in 293T cells. Control, T24 cells transfected with WT or MT FZD4 plasmid, without miR-101 mimics; NC, T24 cells transfected with WT or MT FZD4 plasmid and scramble miR mimics. **P<0.01 vs. control. (D) RT-qPCR was performed to examine the expression of miR-101 in T24 cells transfected with miR-101 inhibitor or NC inhibitor. **P<0.01 vs. NC inhibitor. (E) RT-qPCR and (F) western blot analysis were conducted to examine the mRNA and protein expression levels of FZD4 in T24 cells transfected with miR-101 mimic, miR-NC, miR-101 inhibitor or NC inhibitor. **P<0.01. FZD4, frizzled class receptor 4; miR, microRNA; WT, wild type; MT, mutant; UTR, untranslated region; miR-NC, scramble miR mimics; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; NC, negative control.