Effect of green beans coffee and roasting beans coffee in HFD-fed mice. (A) Body weights of HFD-fed mice treated with coffee brew from green beans or roasted beans on day 0 and day 49. (B) GSH and (C) AsA levels were measured for HFD-fed mice. GSH and AsA levels are measured by using DTNB and DCPIP, respectively. All results are expressed as the mean ± standard error of the mean. Triplicate determinations were performed in each experiment (n=6 in each group). *P<0.05. HFD, high-fat diet; GSH, glutathione; AsA, ascorbic acid; DTNB, dithionitrobenzene; DCPIP, 2,6-dichlorophenolindophenol.