Fig. 1.

Agonist-dependent platelet activation via GpVI or TP, but not PARs elevates [Zn ²⁺ ] _i . Fz-3-labelled washed human platelets were stimulated by CRP-XL ( A ), U46619 ( B ) or thrombin ( C ) and [Zn ²⁺ ] _i fluctuations were monitored over 6 minutes using fluorometry. ( A ) Fz-3 responses to ○ 1 µg/mL, □ 0.3 µg/mL, ▵ 0.1 µg/mL, ⋄ 0.03 µg/mL CRP-XL or • vehicle (DMSO). ( B ) Fz-3 responses to ○ 10 µM, □ 3 µM, ▵ 1 µM, ⋄ 0.3 µM U46619 or • vehicle (DMSO). ( C ) Fz-3 responses to, ○ 1 U/mL, □ 0.3 U/mL, ▵ 0.1 U/mL, ⋄ 0.03 U/mL thrombin or • vehicle (DMSO). Data are mean ± standard error of the mean (SEM) from at least 8 independent experiments. Significance is denoted as *** p  < 0.001, ** p  < 0.01 or * p  < 0.05.