Fig. 5.

Ionophore-induced shape change is sensitive to pre-treatment with (Cyt-D) or TPEN. ( A ) Representative aggregometry traces showing clioquinol (Cq)-, pyrithione (Py)- or A23187-induced (30 µM) shape change following pre-treatment with TPEN (50 µM) or Cyt-D (10 µM). ( B ) Quantitation of minimum aggregation following treatment of platelets pre-treated with TPEN (▪ 25 µM), Cyt-D (▪ 10 µM) or vehicle (□ DMSO, prior to stimulation with Cq, Py or A23187 (30 µM). Data are mean ± standard error of the mean (SEM) of at least 6 experiments. Significance is denoted as *** p  < 0.001, ** p  < 0.01 or * p  < 0.05.