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. 2019 Jan 3;25:77–86. doi: 10.12659/MSM.912929

Figure 2.

Figure 2

PI3K/Akt/mTOR signaling was hyper-activated in MCF-7-P cells and mTOR inhibitor everolimus attenuated MCF-7-P cells stemness. (A) Expression of p-Akt and p-mTOR was detected in MCF-7 and MCF-7-P cells. (B, C) Expression of stemness markers ALDH1 and Nanog was examined MCF-7-P cells with different concentrations of everolimus treatment. (D) p-mTOR expression was measured in MCF-7-P cells with different concentrations of everolimus treatment. (E) ALDH1 activity was determined in cells depicted in (D). (F, G) The cell spheroid formation ability was evaluated in cells depicted in (C). Data were presented as mean ± standard deviation; * P<0.05, ** P<0.01 versus control.