Figure 4.

Everolimus inhibits MCF-7-P spheroids viability, migration, epithelial-mesenchymal transition (EMT), and self-renewal ability. (A) MCF-7-P spheroids were digested and seeded into 96-well plates, followed by different concentrations of everolimus treatment. Then cell viability was determined by MTT assay. (B) Migration ability was detected in MCF-7-P spheroids with different concentrations of everolimus. (C, D) Expression of EMT markers (E-cadherin and vimentin) was measured in MCF-7-P spheroids with different concentrations of everolimus. (E, F) The ability of cells spheroid formation was examined in MCF-7-P spheroids with different concentrations of everolimus. Data were presented as mean ± standard deviation; * P<0.05, ** P<0.01 versus control.