Figure 5.

Everolimus attenuated and enhanced palbociclib sensitivity of MCF-7 cells. (A) MCF-7-P cells were treated with palbociclib with or without everolimus, and followed by cell viability assay. (B) Expression of the apoptosis executors, cleaved caspase-3 and cleaved PARP, was detected in cells depicted in (A). (C, D) Expression of drug resistance genes ABCG2 and MDR1 was examined in cells depicted in (A). (E) MCF-7 cells were treated with palbociclib with or without everolimus and followed by detecting cell viability after 24, 48, and 72 hours. (F) Expression of the executors cleaved caspase-3 and cleaved PARP was detected in cells depicted in (E). Data were presented as mean ± standard deviation; ** P<0.01 versus control.