Evolutionary hypothesis of feeding behavior using species trees reconstructed from the 40 nuclear loci. (A) Feeding behavior reconstruction based on ML topology; (B) Feeding behavior reconstruction based on the ASTRAL species tree. The most likely ancestral feeding behavior reconstruction and alternative reconstructions with a likelihood > 5% is indicated on each branch. The probability (P) of the most likely ancestral state is provided for each node. Clade A represents the tripunctata and cardini group species; Clade B represents the testacea and bizonata group species, and Clade C represents a monophyletic quinaria group, with C1 and C2 representing the two divergent clades within this group; * represents Node 1, the ancestor of the immigrans-tripunctata radiation; ** represents Node 2, the ancestor of the ingroup taxa. Within D. subquinaria, the Coastal and Inland samples are designated by C and I, respectively.