Skip to main content
. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Virology. 2018 Jul 26;522:244–259. doi: 10.1016/j.virol.2018.06.018

Fig. 3. VSVΔG-CHIKV replicates in mouse and human glioblastoma cells.

Fig. 3.

A. Monolayer cultures of human glioblastoma cells were infected with VSVΔG-CHIKV at an MOI of 0.02 (primary inoculation). Two hours later, virus inoculum was removed and cells were washed 3 times with PBS. 24 hrs later, all glioblastoma cell types were robustly infected, as indicated by immunofluorescent labeling with the VSV antibody. Cell nuclei are labeled blue with Hoechst33342 dye. To test for viral propagation, media was collected from these cultures and filtered (0.22 μm filter) before transferring to fresh cultures of uninfected cells (secondary inoculation). 24 hrs later, immunofluorescent labeling indicates infectious transmission of viral progeny generated by cultures during the primary inoculation. B. The same experiment performed using mouse CT-2A cells and primary mouse glia. Scale bar 100 μm. C. and D. The bar graph shows the percentage of infected cells. Values are reported as the mean ± SEM; n = 6. *p < 0.05, * *p < 0.01 vs. normal cells; one-way ANOVA with repeated measures.