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. 2018 Jul 20;56(1):10–17. doi: 10.1136/jmedgenet-2018-105405

Figure 2.

Figure 2

Cyclisation of 019-CAR and eGFP transgene fragments. (A) Agarose gel electrophoresis of PCR fragments, and minicircle ligation products before and after T5 exonuclease treatment. (B) Cyclisation efficiencies of eGFP and 019-CAR transgene amplified with 1 pair of primers or 96 pairs of primers. Unpaired multiple two-tailed t-test. The asterisks in (B) indicate significant differences between 96 pairs of primers group with 1 pair of primers in each different DNA concentration: **p<0.01, ***p<0.001.