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. 2018 Dec 26;11:305–317. doi: 10.1016/j.isci.2018.12.021

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© 2018 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Yeast Srv2 Interacts with Mitochondrial Fission GTPase Dnm1

(A) Phylogenetic tree of Srv2/CAP proteins in different organisms.

(B) Srv2 was fused with the TAP tag, and Dnm1 was tagged with HA epitope. Following calmodulin-affinity chromatography, Srv2-Dnm1 co-precipitation was revealed by immunoblotting for HA. Porin was used as negative control. Note that the Srv2 antibody is less sensitive to Srv2-TAP than Srv2.

(C) Yeasts transformed with DNM1-GFP1∼10 + GFP11, GFP1∼10 + SRV2-GFP11, or DNM1-GFP1∼10 + SRV2-GFP11 were examined using the BiFC assay. mt-DsRed was expressed to label mitochondria. The BiFC signal of SRV2-GFP11 and DNM1-GFP1∼10 revealed the specific interaction of Srv2 with Dnm1 on mitochondria in living cells. Scale bars represent 5 μm.