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. 2019 Jan 7;12:423–432. doi: 10.2147/OTT.S181914

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© 2019 Li et al. This work is published and licensed by Dove Medical Press Limited

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CASC9 interacted with 125a-3p.

Notes: (A) miR-125a-3p binding sites in CASC9 were predicted by bioinformatics analysis. (B) Luciferase reporter assays were performed using HDECs cells cotransfected with the miR-125a-3p mimics and CASC9-wt or CASC9-mut reporter plasmid. (C) Analysis of miR-125a-3p expression levels in HDECs cells transfected with CASC9 overexpressing plasmid or siCASC9 was performed by RT-PCR. (D) Pearson’s correlation analysis was used to determine the correlations between the levels of CASC9 and miR-125a-3p in HAs (n=43). Data are presented as the mean ± SD. ***P<0.001.

Abbreviations: HA, hemangioma; HDEC, HA-derived endothelial cell; Mut, mutation; RT-PCR, reverse transcription-polymerase chain reaction; wt, wild type.