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Hyperglycemia induces aortic oxidative stressin vivo. A) Frozen aortic sections were incubated with 5 μmol/l DHE for 20 min and analyzed using fluorescence microscopy (original magnification ×40). B) Quantification of the fluorescence intensity of ROS levels in the aorta. n = 4. C) Quantification of 8-hydroxy-desoxyguanosine (8-OHdG) levels in the aorta. n = 4. *P < 0.05 vs. Control; #P < 0.05 vs. HFD-control.
