Fig. S3.

HSP22 reduces hyperglycemia-induced endothelial injuryin vitro. HUVECs were transfected with plasmids containing HSP22 or a dominant-negative form for 48 h and then stimulated with high glucose for 24 h. A) HSP22 protein expression was determined by Western blotting. GAPDH was used as a loading control. n = 3. B) Cells in 96-well plates were stained with a Cell Counting Kit-8 (CCK8) to evaluate cell viability. n = 3. C) Cytotoxicity was measured by LDH release in the cell media. n = 3. D) Adhesion of nonstimulated primary human peripheral mononuclear cells (PBMCs) to stimulated HUVECs was quantified. n = 3. E) mRNA expression of ICAM-1 and VCAM-1 was determined by RT-PCR. n = 3. F) Endothelial cell activation-related cytokines were examined by RT-PCR. n = 3. ^*P < 0.05 vs. NC-NG; ^#P < 0.05 vs. NC-HG.