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HSP22 deficiency offsets MitoTEMPO-inhibited hyperglycemia-induced mitochondrial fission in endothelium. ATP was measured in cell homogenates. n = 3. B) Representative immunoreactive bands of DRP1, Ser637 phosphorylated DRP1 (p-DPR1) and GAPDH. C) Quantification of the densitometry of the immunoreactive bands of DRP1/p-DPR1. GAPDH was used as a loading control. n = 3. *P < 0.05 vs. NC-NG; #P < 0.05 vs. HG-MitoTEMPO.
