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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: J Allergy Clin Immunol. 2018 Oct 25;143(1):245–257.e6. doi: 10.1016/j.jaci.2018.10.018

FIG 1.

FIG 1.

Enhanced In vivo polarization of Gimap5-deficent mouse and human CD4+ T cells. (A) Number of CD4+ T cells in the spleen of 8wk WT (black bars) and Gimap5sph/sph (red bars) mice. (B) frequency of splenic CD4+ T cells expressing Foxp3, Tbet, Gata3, or RORγt (n=7). (C-G) Expression of IFNγ, IL-17A, and/or IL-13 in splenic CD4+ T cells after PMA/ionomycin stimulation (n=4). (H) Number of CD4+ T cells in the mesenteric lymph node of 8wk mice. (I) frequency of CD4+ T cells expressing Foxp3, Tbet, Gata3, or RORγt in the mesenteric lymph node (n=7). (J-N) Expression of IFNγ, IL-17A, and/or IL-13 in CD4+ T cells from the mesenteric lymph node after PMA/ionomycin stimulation (n=4). Bars represent mean values ± SD. Statistical significance is determined by Student’s two-tailed test. (O-S) Frequency of CD4+ T cells producing IFNγ, IL-17A, and/or IL-13 upon PMA/ionomycin stimulation. T cells isolated from the PBMCs of a GIMAP5 patient (GIMAP5204-Pro/Pro), his heterozygous parent, and unrelated controls (n=2, 2, and 7, respectively). *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001