FIG 3.

Preferential survival of Gimap5^sph/sph CD4⁺ T cells in T_H17 and iTreg polarizing conditions is associated with TGFβ. (A-B) Proliferation and survival of naïve CD4⁺ T cells from WT and Gimap5^sph/sph mice stimulated 3d in non-polarizing conditions (T_H0) or T_H1, T_H2, T_H17, or iTreg polarizing conditions. Blue histograms represent dead cells; red histograms show live cells. Hatched bars represent non-proliferated cells; solid bars represent proliferated cells. (C) Proliferation of live/dead CD4⁺ T cells after 3d stimulation in different T_H17 polarizing conditions. (D) γH2AX expression (DNA damage) in live CD4⁺ T cells in cycle after 3d stimulation in different polarizing conditions. Data represent mean values ± SD (n=4). All experiments were performed at least three times. Statistical significance is determined by ANOVA followed by Sidak’s multiple comparisons test. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001