FIG 5.

Gimap5^sph/sph mice exhibit increased AHR, CD4⁺ T cell polarization, and airway pathology upon HDM sensitization. C57BL/6 WT (black bars) and Gimap5^sph/sph (red bars) mice were administered 25 μg HDM 9 times IT over three weeks. (A) Airway hyper-responsiveness (AHR) in response to methacholine challenge 1 day after the last HDM challenge (saline: n=3; HDM: n=5–6). (B-D) Number of macrophages, neutrophils, and eosinophils in the BAL fluid (saline: n=3; HDM: n=4–7). (E-F). Number of (E) total lung-infiltrating cells and (F) CD4⁺ T cells (in 2 lobes). (G-J) Percentage of lung-infiltrating CD4⁺CD3⁺ T cells expressing IL-13, IL-17A, IL-13 and IL-17A, or IFNγ upon restimulation with PMA/ionomycin for 6h (saline: n=3; HDM: n=6–7). (K-L) Representative (K) H&E and (L) PAS images of lung tissue from WT and Gimap5^sph/sph mice given saline or HDM. Data represents mean values ± SD. Statistical significance is determined by ANOVA followed by Sidak’s multiple comparisons test. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001