Figure 1. Single-cell gene expression projects Myt1-expressing endocrine progenitors preferentially towards the β-cell fate.

(A-F) p-Creode trajectory analysis of scRNA-seq data from E14.5 PPCs depicting α-, β-, ε-, and γ-cell differentiation from Neurog3⁺ progenitors. Overlays represent gene expression levels on a variance normalized (Asinh) scale. Shown are representative graphs from N=100 resampled runs on n=2 biological replicates. (G) Expression of Neurog3, Ins1, and Gcg plotted as a function of progression on p-Creode trajectories in A. X-axis represents cell states traversed from progenitor to differentiated cell states. Y-axis represents gene expression levels on a variance normalized (Asinh) scale. Dotted lines represent the bifurcation point from a common progenitor (left) and cells projecting into β- (top) or α-branches (bottom) (right). (H) Myt1 expression in β- (top, 306 cells) and α- (bottom, 114 cells) trajectory as they exit the common progenitor pool and progress toward β- and α-cell fates. (I) Integrated expression of selected markers in cells of α- or β-biased trajectories, presented as per cell levels only including cells after the bifurcation point. Error bars, SEM from n=5 p-Creode trajectories generated by resampled runs. ** p<0.01, **** p<0.0001 by t-test. (J) Number of genes expressed in different sub-cell populations at high levels. Only genes with levels >0.1% of the total transcripts were counted. Error bars, SEM. Also see Figure S1 and Table S1.