REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies (with fold of dilution indicted)
488-donkey anti Guinea Pig (1:1000)	Jackson Immunoresearch	706-545-148 RRID:AB_2340472
488-donkey anti goat igG (1:1000)	Jackson Immunoresearch	705-545-147 RRID:AB_2336933
Alexa Fluor® 647 AffiniPure Donkey Anti-Guinea Pig IgG (H+L) (1:1000)	Jackson Immunoresearch	706-605-148 RRID:AB_2340476
Alexa Fluor® 488 AffiniPure Donkey Anti-Rabbit IgG (H+L) (1:1000)	Jackson Immunoresearch	711-545-152 RRID:AB_2313584
Alexa Fluor® 647 AffiniPure Donkey Anti-Rabbit (1:1000)	Jackson Immunoresearch	711-605-152 RRID:AB_2492288
Alexa Fluor® 647 AffiniPure Donkey Anti-Mouse IgG (H+L) (1:1000)	Jackson Immunoresearch	715-605-150 RRID:AB_2340862
Alexa Fluor® 594 AffiniPure Donkey Anti-Mouse (1:1000)	Jackson Immunoresearch	715-585-150 RRID:AB_2340854
Alexa Fluor® 594 AffiniPure Donkey Anti-Goat IgG (H+L) (1:1000)	Jackson Immunoresearch	705-585-003 RRID:AB_2340432
Alexa 647 AffiniPure Rabbit Anti- Syrian Hamster IgG (H+L) (1:1000)	Jackson Immunoresearch	307-605-003 RRID:AB_2339601
Biotin-sp anti rabbit (1:1000)	Jackson Immunoresearch	711065-152 RRID:AB_2340593
biotin anti Rat (1:1000)	Jackson Immunoresearch	712-065-151 (RRID: N/A)
Cy3-Donkey anti-mouse (1:1000)	Jackson Immunoreserach	715-165-150 RRID:AB_2340813
Cy3-streptavidin (1:1000)	Vector laboroatories	SA-1300 (RRID: N/A)
Cy5- streptavidin (1:1000)	Vector laboroatories	SA-1500 (RRID: N/A)
FITC anti Rabbit (1:1000)	Jackson Immunoresearch	711-095-152 RRID:AB_2315776
Goat anti-Pdx1 (1:2000)	ABcam	AB47383 RRID:AB_2162359
Goat anti-somatostatin (1:500)	ABcam	AB30788 RRID:AB_778010
Goat anti-insulin (1:500)	Santa Cruz	sc-7839 RRID:AB_2296108
Guinea pig anti insulin (1:1000)	Dako	A0564 RRID: N/A
Rabbit anti-Myt1 (1:1000)	This paper	N/A
Mouse anti-glucagon (1:5000)	Millipore	MabN238 RRID:NA
Goat anti-Neurog3 (1:1000) Rabbit anti-Pdx1 (1:5000)	This lab	NA
	ABcam	AB47267 RRID:AB_777179
Rabbit anti-Nkx6.1 (1:5000)	Gift of P. Serup	NA
Mouse anti-chicken Pax6(1:50)	Hybridoma bank	AB_528427 RRID: N/A
Rabbit anti-MafB (1:1000)	Gift of R. Stein	NA
Rabbit anti-Cleaved Caspase 3 (1:500)	SYSY	#105173 RRID:AB_887838
Rabbit anti-Ghrelin (1:1000)	Antibody-Online	ABIN223664 RRID:AB_10844168
Rabbit anti-glucagon (1:100)	ABcam	AB92517 RRID:AB_10561971
Rabbit anti-somatostatin (1:2000)	ABcam	AB6741 RRID:AB_955424
Rabbit anti-Ki67(1:500)	Abcam	Ab 15580 RRID:AB_443209
Guinea pig anti-Ppy (1:5000)	Millipore	4041-01 RRID:AB_433709
Rabbit anti-Gastrin (1:500)	Antibody-Online	ABIN734318 RRID:AB_11209678
Bacterial and Virus Strains
Bl-21	Vanderbilt MPB Core	NA
DH5-alpha	Vanderbilt MPB Core	NA
Xl-1	Vanderbilt MPB Core	NA
Biological Samples
Bovine serum albumin	Sigma	A9418
Donkey Serum	Jackson Immunoresearch	017-000-121 RRID:AB_2337258
Fetal Bovine Serum	Atlanta Biologicals	S10250
Chemicals, Peptides, and other key reagents
Collagenase from Clostridium histolyticum	Sigma	C5138
DMSO	Sigma	D2650
Doxycycline	Sigma	D9891
Dapi	Sigma	D9542
Adenosine, periodate oxidized	Sigma	7154
5-Azacytidine	Sigma	A2385
Paraformaldehyde	Sigma	P6148
Millicell	Millipore	PIHA01250
Trypsin	Sigma	C5138-5G
SuperScript III Reverse Transcriptase	Thermo	18080085
RNaseOUT Recombinant Ribonuclease Inhibitor	Thermo	10777019
Barcoded hydrogel microspheres (BHM)	1cell
Droplet Stabilization Oil	Droplet Genomics	DG-DSO-20
Cell Barcoding Chip V2	Droplet Genomics	DG-CBC2-80
1H,1H,2H,2H-perfluoro-1-octanol, 97% ; used at 20% in HFE	Sigma	370533-5g
Exonuclease I	NEB	M0293L
FastDigest HinFI restriction endonuclease	Thermo	FD0804
Agencourt AMPure XP magnetic beads	Beckman Coulter	A63881
Agencourt AMPure XP magnetic beads	Beckman Coulter	A63881
NEBNext® mRNA Second Strand Synthesis Module	NEB	E6111L
HiScribe T7 High Yield RNA Synthesis Kit	NEB	E2040S
RNA Fragmentation Reagents	Thermo	AM8740
PrimeScript Reverse Transcriptase	Takara	2680B
Deoxynucleotide (dNTP) Solution Mix (10 mM each)	NEB	N0447L
RNaseOUT Recombinant Ribonuclease Inhibitor	Thermo	10777019
Kapa 2x HiFi Hot Start PCR mix	VWR	KK2601
Eva Green Dye	VWR	31000-T
Corning Costar Spin-X	Thermo	CLS8162
Qiagen genomic DNA isolation	Qiagen	69504
EZ DNA Methylation Lightning kit	Zymo	D5030
Tn5 enzyme for methylome DNA tagmentation	Home made
10X Ampligase Buffer for methylome library construction	Epicentre	A1905b
dNTP for methylome library construction	Thermo	R72501
T4 DNA Polymerase	NEB	M0203S
Ampligase	Lucigen	A3210K
DNA oligos used (Synthesized by IDT)
Ai9-1	GCGGCCACTACCTGGTGGAG T	Ai9 genotyping
Ai9-2	CCACGCCACGTTGCCTGACAA
ngn3ncre5s	CCAAAGGGTGGATGAGGGG CG	Ngn3nCre genotyping
ngn3ncre5a	ATGTGGCGTCCACGGGGAGT
myt1cCre5s	GGCAAACTTCTGACCCAGAG GT	Myt1cCre genotyping
myt1cCre5a	GTTGGTCCATCCGCCAGCCTG CA
DNMT1	TACCCACCATGACAGGAAGA	Pdx1-DNMT1
DNMT2	GGGATGAGGGTGTTGAACTG	genotyping
3bs	CCCTGACCTACGGCGTGCAG TGC	TetO-Dnmt3b genotyping
3ba	CTCGATGTTGTGGCGGATCTT G
P5	TTGAAACAAGTGCAGGTGTT CG	Pdx1-dCas9 genotyping
1843	CCATCTTATCGTCATCGTCTTT G
rTTA-s	CCAGAAGCTTGGTGTAGAGC AG	RiprTTA genotyping
rTTA-a	GGCTGGCTCTGCACCTTGGT GATC
P5	TTGAAACAAGTGCAGGTGTT CG	Pdx1-Cre genotyping
ngn3ncre5a	ATGTGGCGTCCACGGGGAGT
801	TCCACCTGTCCGTTCAGTG	Myt1F genotyping
806	AGATCCTTCCAGGGTGGAGA
858	GTATGGGGAAACTGCTGAAT GAA	Myt1L genityping
661	GCATCCAGACAGACTGCGGT GA
Myt3B	TGAGACTGAGACTACTTGTTA GC	Myt3F/F genotyping
Myt3C	GCTTTCTGGGTTCATTTCTG
ngn3ncre5s	CCAAAGGGTGGATGAGGGG CG	Ngn3-Myt1 genotyping
Myt1a	GGTAACCCTCATCCAGAGCC AG
eGFPa	GCTGAACTTGTGGCCGTTTAC GT	Ngn3-eGFP genotyping
Ngn3c	TGCAGTGACCTCTAAGTCAG AGGCT
3bs	CCCTGACCTACGGCGTGCAG TGC	Dnmt3t genotyping
3ba	CTCGATGTTGTGGCGGATCTT G
Arxs	ACGACTTCCTCCAGGACTATA C	Arx real time RT-PCR
Arxa	GGCTGTCACCAACTAC TTCAA
Gcgs	TGCAATGGTTAATGAGCACT AAAAG	Gcg real time RT-PCR
Gcga	GATCCGGGAATTTGTCATTCT C
UR-1086	GGATTTATTTTTATTTTATTTT TTGTGTGTG	UR2 CpG island PCR
UR-1215	TTTCCCTCAAACTTCCAAACA AAAACCCTC
UR-1818	GTGTGGAGTTTATTTTGAAGT TA
UR-1819	CAAACTTCCAAACAAAAACCC TC
Pax4-1083	GAAATTGGTATTATTTTTTTTA AAAGGTGTTAAGAG	Pax4 CpG island PCR
Pax4-1041	TAATAATTAAAATTCCTCCCT ACTTCCTTC
Pax6-1078	GTGGTTATGTTTATTAGGTGG TGATTTTA	Pax6 CpG island PCR
Pax6-1089	CTCATTAAAACCAAATAATAA TAAAAAC
Pdx1-1278	TAGATTATTTGTGAGGGTTAA TATTTTGT	Pdx1 CpG island PCR
Pdx1-1279	CCTCAATAAATCCATTATTCA AAATAACC
Myt1-1079	AAGGGTTTATGGGTAGTGTA TTTATAAAG	Myt1 CpG island PCR
Myt1-1007	TAAATTTCAATTAAATATCTTC TCCCCTCA
Nkx2.2-997	TGGAGTTTTAGTTTGAAGTTG GGAGGAGGG	Nkx2.2 CpG island PCR
Nkx2.2-1085	AAAACTCTAAACTCCCAACTC CTTCTACAACCC
Nkx6.1-1080	AAGGTGTGGTGTTTTTAGGT GGGTGTGTTTTAGGAG	Nkx6.1 CpG island PCR
Nkx6.1-1081	AAAAAAAATAAAAAACCAAA ATAAAAACTTTC
Tn5mC-Apt1	T/iMe-dC/GT/iMe-dC/GG/iMe- dC/AG/iMe-dC/GT/iMe- dC/AGATGTGTATAAGAGA/iMe -dC/AG	Methylation assay adaptor
Tn5mC1.1-A1block	5Phos/CTGTCTCTTATA CA/3ddC
Critical Commercial Assays
High capacity cDNA synthesis	Applied BIosystems	4368814
SYBER Green qpcr mix	Biorad	1705060
Deposited Data
Methylome of Ngn3+/− cells (read 1)	1747-GG1-AGGCAGAAAT- TATCCTCTTC_S1_R1_001. fastq.gz	GSE119110- SM3358197
Methylome of Ngn3+/− cells (read 2)	1747-GG1-AGGCAGAAAT- TATCCTCTTC_S1_R2_001. fastq.gz	GSE119110- GSM3358197
Methylome of Ngn3−/− cells (read 1)	1747-GG2-TCCTGAGCAT- AGAGTAGATC_S2_R1_00 1.fastq.gz	GSE119110- GSM3358198
Methylome of Ngn3−/− cells (read 2)	1747-GG1-TCCTGAGCAT- AGAGTAGATC_S2_R2_00 1.fastq.gz	GSE119110- GSM3358198
ScRNAseq-Ngn3+/−___sample one	431-GG-1	GSE118122 GSM3305228
ScRNAseq-Ngn3+/−___sample two	431-GG-2	GSE118122 GSM3305229
ScRNAseq-Ngn3−/−___sample one	431-GG-3	GSE118120
ScRNAseq-Ngn3−/−___sample two	431-GG-4	GSE118120