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. 2018 Dec 27;34(1):350–360. doi: 10.1080/14756366.2018.1553166

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© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Kinetic characterisation of SSBb as hAKR1B1 inhibitor. Panel A and Panel C are the Hanes–Woolf plots obtained when the activity of the purified enzyme (8 mU) was measured at the indicated concentrations of the substrate, in the absence (■) or in the presence of the following inhibitor concentrations: (●) 17.8 µM, (▴) 35.5 µM, (×) 89 µM. Panel B and Panel D refer to the secondary plots of the slopes (1/^appV_max) and the ordinate intercept (^appKM/^appV_max) of the relative Hanes–Woolf plot, as a function of the inhibitor concentration. Panel A and Panel B refer to l-idose; Panel C and Panel D refer to HNE. Error bars (when not visible are within the symbols size) represent the standard deviations of the mean from at least three independent measurements.