Figure 1. SDF-1α/CXCR4 expression was observed in retinal ECs in APB5-treated mouse retina.

(A) The effect of APB5 on Sdf1a mRNA expression in P5, P7, and P9 mouse retinas (n = 6). (B) Representative images of retina in situ hybridization of Sdf1a (purple) and collagen IV (brown) on P8. White arrowheads indicate a double-positive staining region of Sdf1a and collagen IV. Scale bar: 50 μm. (C) Representative images of P8 mouse flow cytometry. Pink circles in left 2 graphs indicate CD11b⁺CD45^hi macrophages. Pink rectangles in right 2 graphs indicate CXCR4-positive macrophages. (D) Representative images of P8 mouse flow cytometry. Pink rectangles in left 2 graphs indicate CD31⁺CD45^– ECs. Pink rectangles in 2 right graphs indicate CXCR4-positive ECs. Significantly different from the results in vehicle-treated mice at *P < 0.05, significantly different from the results in vehicle-treated mice.