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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Gastroenterology. 2018 Aug 23;155(6):1985–1998.e5. doi: 10.1053/j.gastro.2018.08.028

Figure 4: Effect of metavert on its expected targets.

Figure 4:

MIA PaCa-2 cells were cultured for 72h with metavert (1.2μM) or indicated doses. (A) levels of proteins were measured by Western. Membranes were re-probed for GAPDH to confirm equal loading. (B) Maximum intensity projections of confocal images of cells that were immunofluorescent co-labeled for GSK3B (green) and H3K9Ac (red) and counterstained with DAPI (blue). (C) Quantitative results of cell-by-cell high-content analysis of untreated control cells versus metavert-treated cells: Average nuclear intensities (a) and average values for slopes of the co-localization scatter plots (b) of GSK3B and H3K9Ac across all analyzed cells are displayed as bar plots. Representative scatter plots of single cells for GSK3B /DAPI (c) and H3K9 acetylation/DAPI (d). *, p < 0.05 versus control.