Table 3. Loci and primers used for MLVA analysis.
Locus | Primer name | Nucleotide sequence (5′→ 3′) |
Length of STR [bp] | Amplicon size of Nine Mile strain [bp] | No. of STRs for Nine Mile strain | Reference |
---|---|---|---|---|---|---|
Ms23 | Ms23F | FAM-CGCMTAGCGACACAACCAC | 7 | 133 | 9 | Tilburg et al. [4] |
Ms23R | GACGGGCTAAATTACACCTGCT | |||||
Ms24 | Ms24F | FAM-TGGAGGGACTCCGATTAAAA | 7 | 261 | 27 | Tilburg et al. [4] |
Ms24R | GCCACACAACTCTGTTTTCAG | |||||
Ms27 | Ms27F | FAM-TCTTTATTTCAGGCCGGAGT | 6 | 89 | 4 | Klaassen et al. [22] |
Ms27R | GAACGACTCATTGAACACACG | |||||
Ms28 | Ms28F | FAM-AGCAAAGAAATGTGAGGATCG | 6 | 111 | 6 | Klaassen et al. [22] |
Ms28R | GCCAAAGGGATATTTTTGTCCTTC | |||||
Ms33 | Ms33F | FAM-TCGCGTAGCGACACAACC | 7 | 104 | 9 | Tilburg et al. [4] |
Ms33R | GTAGCCCGTATGACGCGAAC | |||||
Ms34 | Ms34F | FAM-TTCTTCGGTGAGTTGCTGTG | 6 | 101 | 5 | Klaassen et al. [22] |
MS34R | GCAATGACTATCAGCGACTCGAA |