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. 2019 Jan 10;14(1):e0210009. doi: 10.1371/journal.pone.0210009

Fig 2. Cytokine production by splenocytes after ex vivo stimulation three weeks post-boost.

Fig 2

Aged (24–26 months) BALB/c mice were immunized twice with H1-VLP, split-virion vaccine or PBS. Three weeks post-boost, splenocytes were collected and stimulated ex vivo for 18 hours with H1-VLP. Flow cytometry was performed to determine the following cell types and cytokine expression: A) CD4+ T cells and B) CD8+ T cells. Subtractive data was used (Stimulated—unstimulated). For statistical analysis, two-way ANOVA was performed (**** p<0.0001, *** p<0.001, ** p<0.01, * p<0.05). Data represent 9–15 mice/group.