Table 1. Primer pair sequences used in real-time RT-PCR [35].
Genes | Oliginucleotides sequence (5’- 3’) |
---|---|
Alkaline phosphatase (ALP) | F: GTTGCCAAGCTGGGAAGAACAC |
R: CCCACCCCGCTATTCCAAAC | |
Osteocalcin (OCN) | F: AGGGAGGATCAAGTCCCG |
R: GAACAGACTCCGGCGCTA | |
Osteopontin (OPN) | F: GACCACATGGACGACGATG |
R: TGGAACTTGCTTGACTATCGA | |
GAPDH | F: CCCTGTTGCTGTAGCCGTA |
R: CCGGTGCTGAGTATGTCG |