Skip to main content
. 2019 Jan 10;14(1):e0210491. doi: 10.1371/journal.pone.0210491

Fig 1. Expression and localization of dvssj1 mRNA at different life stages of western corn rootworm (WCR).

Fig 1

(A) Relative expression of the whole insect of WCR was measured at different life stages by qRT-PCR. Relative expression analysis (mean ± SE) was based on dvssj1 expression in individual insects (n = 12) at each life stage, after being normalized to the expression of the reference gene, dvrps10. Expression data were subjected to one-way analysis of variance using JMP (v12. SAS Institute Inc, Cary, NC) followed by Tukey’s multiple comparison tests. Neonate stage showed statistically significant high expression (P = 0.004[***] or < 0.0001[****]). (B) Visualization of dvssj1 mRNA expression during different life stages by in situ hybridization (ISH). Representative WCR sections were collected from the egg, neonate (neo; first 24-hrs after hatch), 1st, 2nd and 3rd instar of larvae, and dissected testis and ovary from adults. All samples were hybridized with the dvssj1 probe and a control probe (dvrps10) were included for egg, neonate, 2nd and 3rd instars to compare (S2 Fig). Images were captured at 40x magnification with 60 μm scale bars.