Fig 1. CPn0572 influences actin function in S. pombe independently of the ABD-C.

(A) Diagrammatic representation of CPn0572 and CPn0572ΔABD-C with the location of the ABD-C (black) and ABD (white) domain. Numbers indicate amino acid positions. (B) Schematic representation of CPn0572 expression under control of the nmt1⁺ promoter. This promoter is repressed in the presence of thiamine (Low expression) where it shows basal expression and is de-repressed in the absence of thiamine (High expression). (C) Serial dilution patch test (10⁴–10¹ cells) of a wild-type yeast strain transformed with the indicated plasmids and grown for 5 days at 30°C on plasmid selective minimal medium leading to either low expression (Low) or high expression (High) of constructs in the presence of Latrunculin B (LatB) or DMSO solvent control. Reproduced in n = 5 transformants tested per condition.