Fig 5. The C-terminus of CPn0572 binds F-actin in vitro.

(A) Schematic representation of CPn0572 and CPn0572^536-755 variants. ABD-C, black box; ABD, white box; Numbers indicate amino acid positions. (B) and (C) F-actin binding of CPn0572^536-755 was analyzed using an actin co-sedimentation assay. Experiments were performed in the absence (-) or in the presence (+) of 40 μg of pre-assembled F-actin (B and C, respectively). Supernatant (S) and pellet (P) fractions were analyzed by SDS-PAGE and visualized with Coomassie blue. Actin is marked with an arrow and GST-CPn0572^536-755 in the pellet fraction is marked with a (*). n = 2.