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. 2019 Jan 10;14(1):e0210403. doi: 10.1371/journal.pone.0210403

Fig 8. Helix 2 of CPn0572 plays a role in vinculin association.

Fig 8

(A) Schematic representation of CPn0572 and variants thereof. (B) Confocal fluorescence microscopy analysis of transfected cells expressing CPn0572 variants fused to GFP and vinculin fused to SNAP. HEp-2 cells were transfected for 18 h before fixation. Vinculin was visualized with SNAP-cell SiR647 (magenta) and DNA with DAPI (blue). Bars: 10 μm. (C) Schematic representation of the methodology used for the quantification of GFP fluorescence intensity colocalized with vinculin. (D) GFP fluorescence intensity of GFP-CPn0572 variants that colocalize with vinculin puncta. arbitrary units (a.u.). n = 90 independent measurements for each construct analyzed from triplicates.